Coarsened mixtures of hierarchical skew normal kernels for flow cytometry analyses

Understand

Flow cytometry (FCM) is the standard multi-parameter assay for measuring single cell phenotype and functionality.

• It is commonly used for quantifying the relative frequencies of cell subsets in blood and disaggregated tissues.
• A typical analysis of FCM data involves cell classification---that is, the identification of cell subgroups in the sample---and comparisons of the cell subgroups across samples or conditions.
• While modern experiments often necessitate the collection and processing of samples in multiple batches, analysis of FCM data across batches is challenging because differences across samples may occur due to either true biological variation or technical reasons such as antibody lot effects or instrument optics across batches.