DeepSTORM3D: dense three dimensional localization microscopy and point spread function design by deep learning

Understand

Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g.

• fluorescent molecules) are determined at high precision from their images.
• This is the key ingredient in single/multiple-particle-tracking and several super-resolution microscopy approaches.
• Localization in three-dimensions (3D) can be performed by modifying the image that a point-source creates on the camera, namely, the point-spread function (PSF).